From 27-28 June, 2012 the Grand Dorsett Subang Hotel, Kuala Lumpur will play host to the Separation Science 2012, an event that comprises of world-class international and regional speakers, unique troubleshooting panel sessions and an exhibition of state-of-the-art instrumentation and equipment for analytical scientists working across pharmaceutical, food, environmental and clinical markets.
Research Instruments is the local distributor of Waters chromatography and mass spectroscopy products and we are pleased to have two scientists from Waters to discuss the following topics:
Moving Supercritical Fluid Chromatography into the Ultra Performance Arena to Become The Interface of GC and LC separations
Mark Ritchie – Waters Pacific, Singapore, 27th June, 1400 - 1420hrs
Supercritical fluid chromatography (SFC) is a form of normal phase chromatography that utilizes carbon dioxide under super-critical pressures as a mobile phase. With the intermediate properties between liquids and gases, the low viscosity and high diffusivity of supercritical CO2 as a mobile phase component results in higher optimal linear velocities and a lower pressure drop across a column for analyses. It has also been a very attractive technique in the analytical separation of isomers, when used with an appropriate mobile phase. In addition it has been touted as a “green” technique as the solvent use is greatly decreased, and the CO2 for the mobile phase is a by product of other industrial processes.
Recent developments in pumping and fluidic control combined with the application of smaller particle (sub 2 micron) stationary phases and decreased system dispersion have allowed this technique to enter the ultra performance arena. This new analytical ability has been explored in a variety of application areas from biochemical studies through to food safety analysis (e.g. pesticides) for both for chiral and achiral species. Initial investigations have shown good separation of both volatile and non volatile species at a variety of polarities, for example essential oils through to phospholipids and hydrocarbons through to sulphonamides. Many of these species are favored by either GC or LC techniques, lending the term “convergence chromatography” to the interface between these techniques afforded by this extension of SFC.
Improving UPLC Performance for the Routine Analysis of Drug Final Formulations
Doug McCabe (Waters Corp., USA), 28th June, 1000 - 1020hrs
An application area where the sample throughput, analyte response and chromatographic resolution benefits of UPLC technology have translated into significant business productivity gains is the routine analysis of drug final formulations. In this very practical seminar we will describe how UPLC technology can best be utilized in laboratories that follow chromatographic test methods found in compendial monographs. We will provide examples of drug final formulations assays taken from the USP-NF that were successfully transferred to UPLC along with routine analysis studies where the long-term robustness of the UPLC methods was evaluated.
For registration information, please click here.